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recombinant murine r spondin 1  (R&D Systems)


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    Structured Review

    R&D Systems recombinant murine r spondin 1
    Recombinant Murine R Spondin 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+r+spondin/Recombinant+Mouse+R-Spondin+1+Protein%2C+CF/bio_rxiv__64898__2026__02__09__704138-163-49-52
    Average 95 stars, based on 128 article reviews
    recombinant murine r spondin 1 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Obese adipose tissue-derived extracellular vesicles enriched with glycolytic cargo promote colorectal cancer tumorigenesis.
    Article Snippet: Obesity increases the risk of colorectal cancer (CRC) development, accelerates disease progression and is associated with decreased disease-free survival.. Obesity adversely affects the visceral adipose tissue (VAT) leading to increased secretion of extracellular vesicles (EVs).. However, the crosstalk between VAT and CRC tumor cells still remains unclear.

    Article Title: Obese Adipose Tissue Extracellular Vesicles Activate Mitochondrial Fatty Acid β-oxidation to Drive Colonic Stemness
    Article Snippet: Next, colonic crypts were separated from colonic tissue through gentle shaking by hand, visualized under inverted microscope (JENCO), and embedded in Reduced Growth Factor Matrigel (356230, BD Biosciences). .. Colonoids were cultured in WERN media: Advanced DMEM/F-12 (Gibco, 12634-010, ThermoFisher) supplemented with 1% GlutaMAX (Gibco 35050-061, ThermoFisher), 100 μg/mL Primocin (ant-pm-05, InVivogen), HEPES (Gibco 15630-080, ThermoFisher), 1× N2 supplement (Gibco 17502-048, ThermoFisher), 1 ng/μL EGF (2028-EG, R&D Systems), 2 ng/μL Noggin (250-38, PeproTech), 10 ng/μL murine R-spondin (3474-RS-050, R&D Systems), Y27632 (ALX-270-333-M025, Enzo Life Sciences), and 100 ng/mL (standard concentration) or reduced (50, 25, or 0) concentrations of mouse recombinant Wnt3α (1324-WN-002, R&D Systems). .. Complete WERN organoid media was obtained from the Organoid and Tissue Modeling Shared Resource core, University of Colorado Anschutz Medical Campus.

    Article Title: Obese adipose tissue extracellular vesicles activate mitochondrial fatty acid β-oxidation to drive colonic stemness.
    Article Snippet: Next, colonic crypts were separated from colonic tissue through gentle shaking by hand, 507 visualized under inverted microscope (JENCO, USA), and embedded in Reduced Growth Factor 508 Matrigel (356230, BD Biosciences). .. Colonoids were cultured in WERN media: Advanced 509 DMEM/F-12 (Gibco, 12634-010, ThermoFisher) supplemented with 1% GlutaMAX (Gibco 510 35050-061, ThermoFisher), 100 g/ml Primocin (ant-pm-05, InVivogen), HEPES (Gibco 15630-511 Jo urn al Pr e-p roo f 080, ThermoFisher), 1× N2 supplement (Gibco 17502-048, ThermoFisher), 1 ng/μl EGF (2028-512 EG, R&D Systems), 2 ng/μl Noggin (250-38, PeproTech), 10 ng/μl murine R-spondin (3474-RS-513 050, R&D Systems), Y27632 (ALX-270-333-M025, Enzo Life Sciences), and 100 ng/ml 514 (standard concentration) or reduced (50, 25, or 0) concentrations of mouse recombinant Wnt3 515 (1324-WN-002, R&D Systems). .. Complete WENR organoid media was obtained from the 516 Organoid and Tissue Modeling Shared Resource core, University of Colorado Anschutz Medical 517 Campus.

    Isolation:

    Article Title: Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid model.
    Article Snippet: .. The jejunal crypts (day 0) isolated from male 3- to 5-month-old B6 albino mice were grown in Reduced Growth Factor Matrigel (Corning, Corning, NY; 356,231) and advanced DMEM/F12 (Gibco; 12634-028) containing GlutaMAX (Gibco; 35050-061), Primocin (Invivogen, San Diego, CA; ant-pm-05), HEPES (Gibco; cat. 15630-080), N2 supplement (Gibco; cat. 17502-048), 50 ng/μL EGF (R&D Systems, Minneapolis, MN; 2028-EG), 100 ng/μL Noggin (PeproTech, Rocky Hill, NJ; 250 − 38), 250 ng/μL murine R-spondin (R&D Systems, Minneapolis, MN; 3474- RS-050), and 10 μM Y27632 (Enzo Life Sciences, East Farmingdale, NY; ALX270-333). .. For Wnt suppression studies, IWP2 (Tocris Bioscience, Bristol, UK; 3533), the compound that inhibits Wnt processing and secretion, was added on day 0 and day 3.

    Article Title: Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid model
    Article Snippet: .. The jejunal crypts (day 0) isolated from male 3- to 5-month-old B6 albino mice were grown in Reduced Growth Factor Matrigel (Corning, Corning, NY; 356,231) and advanced DMEM/F12 (Gibco; 12634-028) containing GlutaMAX (Gibco; 35050-061), Primocin (Invivogen, San Diego, CA; ant-pm-05), HEPES (Gibco; cat. 15630-080), N2 supplement (Gibco; cat. 17502-048), 50 ng/μL EGF (R&D Systems, Minneapolis, MN; 2028-EG), 100 ng/μL Noggin (PeproTech, Rocky Hill, NJ; 250 − 38), 250 ng/μL murine R-spondin (R&D Systems, Minneapolis, MN; 3474-RS-050), and 10 μM Y27632 (Enzo Life Sciences, East Farmingdale, NY; ALX270-333). .. For Wnt suppression studies, IWP2 (Tocris Bioscience, Bristol, UK; 3533), the compound that inhibits Wnt processing and secretion, was added on day 0 and day 3.

    Concentration Assay:

    Article Title: Obese Adipose Tissue Extracellular Vesicles Activate Mitochondrial Fatty Acid β-oxidation to Drive Colonic Stemness
    Article Snippet: Next, colonic crypts were separated from colonic tissue through gentle shaking by hand, visualized under inverted microscope (JENCO), and embedded in Reduced Growth Factor Matrigel (356230, BD Biosciences). .. Colonoids were cultured in WERN media: Advanced DMEM/F-12 (Gibco, 12634-010, ThermoFisher) supplemented with 1% GlutaMAX (Gibco 35050-061, ThermoFisher), 100 μg/mL Primocin (ant-pm-05, InVivogen), HEPES (Gibco 15630-080, ThermoFisher), 1× N2 supplement (Gibco 17502-048, ThermoFisher), 1 ng/μL EGF (2028-EG, R&D Systems), 2 ng/μL Noggin (250-38, PeproTech), 10 ng/μL murine R-spondin (3474-RS-050, R&D Systems), Y27632 (ALX-270-333-M025, Enzo Life Sciences), and 100 ng/mL (standard concentration) or reduced (50, 25, or 0) concentrations of mouse recombinant Wnt3α (1324-WN-002, R&D Systems). .. Complete WERN organoid media was obtained from the Organoid and Tissue Modeling Shared Resource core, University of Colorado Anschutz Medical Campus.

    Article Title: Obese adipose tissue extracellular vesicles activate mitochondrial fatty acid β-oxidation to drive colonic stemness.
    Article Snippet: Next, colonic crypts were separated from colonic tissue through gentle shaking by hand, 507 visualized under inverted microscope (JENCO, USA), and embedded in Reduced Growth Factor 508 Matrigel (356230, BD Biosciences). .. Colonoids were cultured in WERN media: Advanced 509 DMEM/F-12 (Gibco, 12634-010, ThermoFisher) supplemented with 1% GlutaMAX (Gibco 510 35050-061, ThermoFisher), 100 g/ml Primocin (ant-pm-05, InVivogen), HEPES (Gibco 15630-511 Jo urn al Pr e-p roo f 080, ThermoFisher), 1× N2 supplement (Gibco 17502-048, ThermoFisher), 1 ng/μl EGF (2028-512 EG, R&D Systems), 2 ng/μl Noggin (250-38, PeproTech), 10 ng/μl murine R-spondin (3474-RS-513 050, R&D Systems), Y27632 (ALX-270-333-M025, Enzo Life Sciences), and 100 ng/ml 514 (standard concentration) or reduced (50, 25, or 0) concentrations of mouse recombinant Wnt3 515 (1324-WN-002, R&D Systems). .. Complete WENR organoid media was obtained from the 516 Organoid and Tissue Modeling Shared Resource core, University of Colorado Anschutz Medical 517 Campus.

    Recombinant:

    Article Title: Obese Adipose Tissue Extracellular Vesicles Activate Mitochondrial Fatty Acid β-oxidation to Drive Colonic Stemness
    Article Snippet: Next, colonic crypts were separated from colonic tissue through gentle shaking by hand, visualized under inverted microscope (JENCO), and embedded in Reduced Growth Factor Matrigel (356230, BD Biosciences). .. Colonoids were cultured in WERN media: Advanced DMEM/F-12 (Gibco, 12634-010, ThermoFisher) supplemented with 1% GlutaMAX (Gibco 35050-061, ThermoFisher), 100 μg/mL Primocin (ant-pm-05, InVivogen), HEPES (Gibco 15630-080, ThermoFisher), 1× N2 supplement (Gibco 17502-048, ThermoFisher), 1 ng/μL EGF (2028-EG, R&D Systems), 2 ng/μL Noggin (250-38, PeproTech), 10 ng/μL murine R-spondin (3474-RS-050, R&D Systems), Y27632 (ALX-270-333-M025, Enzo Life Sciences), and 100 ng/mL (standard concentration) or reduced (50, 25, or 0) concentrations of mouse recombinant Wnt3α (1324-WN-002, R&D Systems). .. Complete WERN organoid media was obtained from the Organoid and Tissue Modeling Shared Resource core, University of Colorado Anschutz Medical Campus.

    Article Title: Obese adipose tissue extracellular vesicles activate mitochondrial fatty acid β-oxidation to drive colonic stemness.
    Article Snippet: Next, colonic crypts were separated from colonic tissue through gentle shaking by hand, 507 visualized under inverted microscope (JENCO, USA), and embedded in Reduced Growth Factor 508 Matrigel (356230, BD Biosciences). .. Colonoids were cultured in WERN media: Advanced 509 DMEM/F-12 (Gibco, 12634-010, ThermoFisher) supplemented with 1% GlutaMAX (Gibco 510 35050-061, ThermoFisher), 100 g/ml Primocin (ant-pm-05, InVivogen), HEPES (Gibco 15630-511 Jo urn al Pr e-p roo f 080, ThermoFisher), 1× N2 supplement (Gibco 17502-048, ThermoFisher), 1 ng/μl EGF (2028-512 EG, R&D Systems), 2 ng/μl Noggin (250-38, PeproTech), 10 ng/μl murine R-spondin (3474-RS-513 050, R&D Systems), Y27632 (ALX-270-333-M025, Enzo Life Sciences), and 100 ng/ml 514 (standard concentration) or reduced (50, 25, or 0) concentrations of mouse recombinant Wnt3 515 (1324-WN-002, R&D Systems). .. Complete WENR organoid media was obtained from the 516 Organoid and Tissue Modeling Shared Resource core, University of Colorado Anschutz Medical 517 Campus.



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